EFFECTS ON FITNESS COMPONENTS OF ENHANCED EXPRESSION OF ELONGATION-FACTOR EF-1-ALPHA IN DROSOPHILA-MELANOGASTER .1. THE CONTRASTING APPROACHES OF MOLECULAR AND POPULATION BIOLOGISTS

TitleEFFECTS ON FITNESS COMPONENTS OF ENHANCED EXPRESSION OF ELONGATION-FACTOR EF-1-ALPHA IN DROSOPHILA-MELANOGASTER .1. THE CONTRASTING APPROACHES OF MOLECULAR AND POPULATION BIOLOGISTS
Publication TypeJournal Article
Year of Publication1993
AuthorsStearns, SC, Kaiser M, and HILLESHEIM E
JournalThe American NaturalistThe American Naturalist
Volume142
Pagination961-993
Accession NumberA1993MQ51700005
Abstract

We measured the life spans and fecundities of mated female fruit flies that had enhanced expression at higher temperatures of the gene for elongation factor 1alpha (EF-1alpha), a gene of known physiological function, and compared those measurements with similar measurements made on control lines at 25-degrees and 29.5-degrees-C. We also measured similar effects on the life spans of virgin males and females. The hypothesis that enhanced expression of EF-1alpha at 29.5-degrees-C increases fife span was tested by the significance of the treatment-by-temperature interaction effect in factorial ANOVAs and Cox regressions. This effect was significant for mated females only. Treatment females with enhanced expression of EF-1alpha lived significantly longer and laid significantly fewer eggs early in life than did control females, a result not mediated by possible effects of EF-1alpha on body weight. There was an effect of treatment independent of the interaction with temperature. Virgin treatment females lived significantly longer at both 25-degrees and 29.5-degrees-C. In contrast to previous results, enhanced expression of EF-1alpha had no impact on the life spans of virgin males. Implications are discussed for the evolutionary theory of aging and for biases introduced into experimental design through the influence of particular theories.

Short TitleAm Nat
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